[Purification of oleuropein by sephadex LH-20 column]

To purify the oleuropein crude extracts by sephadex LH-20 column chromatograph.

This experiment used fast protein chromatography system (AKTA FPLC) produced by Amersham, Sweden. The chromatography column (20 mm x 300 mm) was matched with protein purification instrument. Sephadex LH-20 was used in the Fast Protein Liquid Chromatography columns. The mobile phase was 50% ethanol with a flow velocity of 1.0 mL per minute and the detection wavelength was 254 nm. The content of oleuropein was determined by HPLC.

The purity of oleuropein was 82.9% after passing the column twice when the sample volume was 2 mL.

Sephadex LH-20 can be re-used and the regeneration is convenient, it also provides a reference for the production of oleuropein.