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Updated: Jun 12, 2026
11:16
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
Published on: August 7, 2016
The use of markers for correlative light electron microscopy
1Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK.
Protoplasma
|June 5, 2010
Summary
Developing new bioimaging markers is crucial for tracking molecules in cells. This study highlights challenges and strategies for correlative light and electron microscopy (CLEM) probes, emphasizing rigorous testing for intracellular transport research.
Area of Science:
- Cell Biology
- Microscopy Techniques
- Molecular Imaging
Background:
- Bioimaging advances rely on markers for visualizing molecules in cells.
- Correlative Light and Electron Microscopy (CLEM) requires dual-function probes (fluorescent and electron-dense).
- Existing markers often lack compatibility between light and electron microscopy.
Purpose of the Study:
- To discuss strategies and challenges in developing and using CLEM markers.
- To emphasize the critical need for extensive testing of new probes.
- To focus on CLEM marker application in studying intracellular transport and endocytosis.
Main Methods:
- Review of current strategies for creating dual-function bioimaging probes.
- Analysis of limitations of existing fluorescent and electron-dense markers.
- Case study focus on intracellular transport and endocytosis.
Main Results:
- Few markers possess both fluorescence and electron density for CLEM.
- Green fluorescent protein and gold particles are not directly compatible.
- Intensive search for universally visible markers is ongoing.
Conclusions:
- Extensive validation of new CLEM probes is essential before widespread adoption.
- Careful selection and testing of markers are critical for reliable bioimaging.
- Understanding marker limitations is key for advancing the study of cellular processes like endocytosis.
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