Related Experiment Video
Updated: Jun 12, 2026
12:23
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Complete genome sequence of Bacillus thuringiensis mutant strain BMB171
Jin He1, Xiaohu Shao, Huajun Zheng
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, People's Republic of China.
Journal of Bacteriology
|June 8, 2010
Summary
We sequenced the genome of Bacillus thuringiensis mutant strain BMB171. This acrystalliferous strain exhibits high transformation frequency, valuable for biopesticide development.
Area of Science:
- Microbiology
- Genomics
- Biotechnology
Background:
- Bacillus thuringiensis is a well-established biopesticide.
- Acrystalliferous strains are useful for genetic manipulation.
- High transformation frequency facilitates strain improvement.
Purpose of the Study:
- To report the finished and annotated genome sequence of Bacillus thuringiensis mutant strain BMB171.
- To characterize an acrystalliferous mutant with high transformation frequency.
Main Methods:
- Genome sequencing and annotation.
- Bacterial culturing and genetic manipulation.
Main Results:
- The complete genome sequence of B. thuringiensis BMB171 was obtained and annotated.
- Strain BMB171 was confirmed as acrystalliferous and possessing high transformation frequency.
Conclusions:
- The genome sequence provides a valuable resource for understanding B. thuringiensis genetics.
- Strain BMB171's characteristics make it a promising candidate for biopesticide research and development.
Related Concept Videos
01:18Production of Biopesticides
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...