Monoolein cubic phase containing acidic proteinoid: pH-dependent release
1Division of Biotechnology & Bioengineering, Institute of Bioscience and Biotechnology, Kangwon National University, Chunchon, Korea.
Objective:
Monoolein (MO) cubic phase containing acidic proteinoid was prepared for a pH-dependent release.
Methods:
The acidic proteinoid was prepared by a thermal-condensation reaction of Asp and Leu (9.85/0.15 in molar ratio). To prepare MO cubic phase, molten MO was hydrated with the proteinoid solution in distilled water. For pH-dependent release experiment, amaranth was included as an anionic dye, and either auramine O or methylene blue was contained as a cationic dye.
Results:
The release of amaranth from the cubic phase was promoted under neutral and alkali conditions, possibly because of electrostatic repulsions between the anionic dye and the ionized carboxylic group of the acidic proteinoid. On the contrary, the releases of auramine O and methylene blue were suppressed under neutral and alkali conditions, probably because of electrostatic interactions between the cationic dyes and the ionized carboxylic group.
Conclusion:
The acidic proteinoid is believed to control the releases in response to change in pH.
Insights
This study developed a pH-sensitive monoolein (MO) cubic phase incorporating an acidic proteinoid. The proteinoid controlled the release of anionic and cationic dyes based on pH-induced electrostatic interactions.
Area of Science:
- Materials Science
- Biochemistry
- Drug Delivery Systems
Background:
- Monoolein (MO) cubic phases are advanced lipidic self-assembled systems with potential in controlled release applications.
- Acidic proteinoids, derived from amino acids, offer tunable properties for biomaterial development.
- Understanding pH-responsive mechanisms is crucial for designing targeted delivery systems.
Purpose of the Study:
- To prepare a monoolein (MO) cubic phase incorporating an acidic proteinoid for pH-dependent release studies.
- To investigate the influence of pH on the release kinetics of anionic and cationic dyes from the MO cubic phase system.
Main Methods:
- An acidic proteinoid was synthesized via thermal condensation of Aspartic acid and Leucine.
- Monoolein (MO) cubic phases were formed by hydrating molten MO with the proteinoid solution.
- pH-dependent release of amaranth (anionic dye) and auramine O/methylene blue (cationic dyes) was evaluated.
Main Results:
- The release of the anionic dye amaranth was enhanced under neutral and alkaline conditions due to electrostatic repulsion with the ionized proteinoid.
- The release of cationic dyes (auramine O, methylene blue) was inhibited under neutral and alkaline conditions due to electrostatic attraction with the proteinoid.
- These differential release patterns demonstrate pH-controlled modulation by the acidic proteinoid.
Conclusions:
- The incorporated acidic proteinoid effectively modulates the release of charged molecules from the monoolein cubic phase in a pH-dependent manner.
- This pH-responsive behavior suggests potential applications in targeted drug delivery systems where release is triggered by specific physiological pH changes.
Related Concept Videos
00:53Extraction: Effects of pH
01:19Composition of Polyprotic Acid Solutions as a Function of pH
A graph with the alpha values is plotted against the volume of base added during titration. Here, a...
01:14Alkylation of β-Diester Enolates: Malonic Ester Synthesis