Application of isotope dilution ICP-MS techniques to quantitative proteomics

Area of Science:

• Analytical Chemistry
• Biochemistry
• Proteomics

Background:

• Isotope dilution analysis (IDA) is a well-established method for accurate elemental quantification.
• Concepts from elemental speciation, like species-specific and unspecific IDA, are now applied to biomolecules.
• Proteomics requires robust quantification methods for complex biological samples.

Purpose of the Study:

• To review recent developments and applications of ICP-MS based isotope dilution techniques in protein analysis.
• To critically assess the potential of these techniques for absolute protein quantification.
• To highlight the growing importance of ICP-MS IDA in proteomics.

Main Methods:

• Isotope dilution analysis (IDA) coupled with inductively coupled plasma mass spectrometry (ICP-MS).
• Application of species-specific and unspecific IDA principles to protein quantification.
• Utilizing heteroatom-containing proteins and artificial metal-containing labels for detection.

Main Results:

• ICP-MS based IDA enables accurate and precise absolute protein quantification.
• The technique is applicable to both naturally occurring and labeled proteins.
• Demonstrated value in elemental speciation studies transferred to biomolecule analysis.

Conclusions:

• ICP-MS based isotope dilution analysis is an emerging and valuable tool for quantitative proteomics.
• The ability to perform absolute quantification makes it attractive for current and future research.
• Further developments and applications are expected to enhance its role in the field.