Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

961
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
961
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

900
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
900
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

648
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
648
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

629
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
629
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

1.2K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
1.2K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

909
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
909

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell ICP-MS for assessing genetic modifications affecting intracellular phosphorus levels and polyphosphate nanoparticle formation in Streptomyces coelicolor.

Talanta·2026
Same author

Built by Iron, changed by heat: Mineral bioaccessibility in iron-fortified and processed Hermetia illucens larvae.

Food chemistry·2026
Same author

Sequential versus Simultaneous Quantitative Analysis of Biomarkers in Individual Cells by ICP-MS and Mass Cytometry: A Focus on Immunotherapy.

Analytical chemistry·2025
Same author

Deciphering the formation of biogenic nanoparticles and their protein corona: State-of-the-art and analytical challenges.

Analytical and bioanalytical chemistry·2025
Same author

Targeting Intracellular miRNA in Different Cancer Cell Models Using Gold Nanoprobes and Combined Mass Cytometry and Single Particle ICP-MS.

Nano letters·2025
Same author

Quantitative determination of intracellular miRNA content using dual gold and iron nanoreporters and single particle ICP-ToF-MS.

Mikrochimica acta·2025

Related Experiment Video

Updated: Sep 20, 2025

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

11.3K

Single particle and single cell analysis by ICP-MS with pneumatic nebulisation: Comparing sample introduction

Andrés Suárez Priede1, Mario Corte-Rodríguez1, Hannes Gödde2

  • 1Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, E-33006, Oviedo, Spain; Health Research Institute of the Principality of Asturias (ISPA), Av. Hospital Universitario s/n, E-33011, Oviedo, Spain.

Talanta
|May 29, 2025
PubMed
Summary
This summary is machine-generated.

Accurate elemental analysis using single particle (sp) and single cell (sc) inductively coupled plasma-mass spectrometry (ICP-MS) requires precise transport efficiency (TE) determination. This study evaluated nebulizers and spray chambers, finding TE varies significantly with different sample types.

Keywords:
ICP-MSPneumatic nebulisationSingle cellSingle particleTransport efficiency

More Related Videos

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS

Published on: July 11, 2017

10.9K
In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

7.2K

Related Experiment Videos

Last Updated: Sep 20, 2025

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

11.3K
A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS

Published on: July 11, 2017

10.9K
In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

7.2K

Area of Science:

  • Analytical Chemistry
  • Nanomaterial Characterization
  • Cellular Analysis

Background:

  • Inductively coupled plasma-mass spectrometry (ICP-MS) in single particle (sp) and single cell (sc) modes is crucial for elemental analysis of nanomaterials and cells.
  • Pneumatic nebulization is the preferred sample introduction system for ICP-MS.
  • Accurate quantitative data in sp- and sc-ICP-MS relies heavily on the precise determination of transport efficiency (TE).

Purpose of the Study:

  • To investigate the performance of various commercial nebulizer and spray chamber combinations for sp- and sc-ICP-MS.
  • To evaluate the impact of different sample introduction systems on transport efficiency.
  • To determine the influence of various sample types on TE measurements.

Main Methods:

  • Testing of multiple nebulizer and spray chamber configurations for ICP-MS.
  • Utilizing high sample flow rates (0.4 mL/min) with a traditional cyclonic spray chamber.
  • Employing specialized total consumption spray chambers (Cytospray and HE-SIS) at optimal flow rates (10 μL/min).
  • Determining transport efficiencies using the particle number method with three model suspensions: gold nanoparticles, europium-loaded polystyrene beads, and selenized yeast.

Main Results:

  • Higher sensitivities (approximately 5-fold) were achieved with high consumption setups compared to traditional ones.
  • Transport efficiencies varied significantly (up to 90%) depending on the nebulizer-spray chamber configuration and the type of model suspension used.
  • Observed differences in TE among gold nanoparticles, polystyrene beads, and yeast highlight the critical role of sample-specific calibration.

Conclusions:

  • The choice of nebulizer and spray chamber significantly impacts achievable sensitivity and transport efficiency in sp- and sc-ICP-MS.
  • Accurate TE determination necessitates the use of a suitable calibrant that matches the sample matrix and particle characteristics.
  • These findings are critical for advancing reliable quantitative elemental analysis in nanomaterial and cellular studies using ICP-MS.