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High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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Matrix-assisted diffusion-ordered spectroscopy: mixture resolution by NMR using SDS micelles.

Claudio F Tormena1, Robert Evans, Stephan Haiber

  • 1School of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.

Magnetic Resonance in Chemistry : MRC
|June 12, 2010
PubMed
Summary
This summary is machine-generated.

Matrix-assisted DOSY (MAD) enhances diffusion-ordered spectroscopy (DOSY) for separating isomers. This study confirms MAD

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Area of Science:

  • Analytical Chemistry
  • Spectroscopy

Background:

  • Diffusion-ordered spectroscopy (DOSY) is vital for mixture analysis.
  • Basic DOSY struggles to separate species with similar diffusion coefficients, like isomers.
  • Matrix-assisted DOSY (MAD) uses co-solutes to perturb diffusion and resolve such mixtures.

Purpose of the Study:

  • To investigate the optimal concentration ranges for matrix-assisted DOSY (MAD) using SDS surfactant.
  • To determine the conditions for achieving diffusion resolution of monomethoxyphenol isomers via MAD.
  • To assess the robustness of MAD separation across varying surfactant and solute concentrations.

Main Methods:

  • Utilized matrix-assisted DOSY (MAD) with sodium dodecyl sulfate (SDS) as the surfactant.
  • Employed a model mixture of monomethoxyphenol isomers.
  • Systematically varied the concentrations of SDS and the isomeric solutes.

Main Results:

  • Demonstrated successful diffusion resolution of monomethoxyphenol isomers using MAD.
  • Found that the separation is highly robust across a wide range of SDS and solute concentrations.
  • Indicated that MAD is effective even with varying concentration ratios of surfactant and solutes.

Conclusions:

  • Matrix-assisted DOSY (MAD) effectively resolves isomers with similar diffusion coefficients.
  • The MAD technique shows remarkable robustness concerning surfactant and solute concentrations.
  • MAD shows promise as a valuable and adaptable tool for complex mixture analysis.