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Updated: Jun 12, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
[Reproducibility of a method to quantify vaginal human immunodeficiency virus viral load]
Adelina Gimeno1, Joaquín Plazas, José Sánchez-Payá
1Laboratorio de Microbiología Molecular, Hospital General Universitario de Alicante, Alicante, España.
Objective:
To assess the reproducibility of a method to collect and quantify HIV nucleic acids in vaginal secretions.
Methods:
We analysed two consecutive vaginal samples collected by direct aspiration from 52 HIV infected women. Nucleic acids were extracted by QIAamp RNA-viral and quantified with a modified Cobas Amplicor HIV-1 Monitor.
Results:
Intra-class correlation coefficient between matched samples: 0.99. Differences of pooled HIV DNA+RNa and RNA were <0.40 uLog for 95% of all samples (Bland-Altman plots).
Conclusions:
This method is a standard and reproducible assay to detect and measure HIV vaginal viral load.
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