Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 12, 2026

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
06:49

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Published on: December 17, 2021

A label-free nanoparticle aggregation assay for protein complex/aggregate detection and study.

Jelena Bogdanovic1, Jimmie Colon, Cheryl Baker

  • 1NanoScience Technology Center, University of Central Florida, Orlando, FL 32826, USA.

Analytical Biochemistry
|June 18, 2010
PubMed
Summary

A new nanoparticle aggregation immunoassay (NanoDLSay) offers a sensitive, label-free method for detecting protein aggregates. This technique enhances biomolecular research and biopharmaceutical development by simplifying protein complex analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harnessing artificial intelligence in gastrointestinal endoscopy for early detection of dysplastic lesions in inflammatory bowel disease.

World journal of gastrointestinal endoscopy·2026
Same author

Stem cells in action: addressing ischemia-reperfusion injury in acute kidney injury.

Molecular biology reports·2025
Same author

Uncommon Factors Leading to Nephrotic Syndrome.

Biomedicines·2025
Same author

Evaluating a Rapid Immunity Test to Predict Dairy Calf Mortality Risk.

Biology·2025
Same author

Incretin-Based Therapies and Cancer: What's New?

Medicina (Kaunas, Lithuania)·2025
Same author

Rapid Isolation and Characterization of Exosomes through a Single-Step, Label-Free Protein Biomarker Analysis.

ACS applied bio materials·2025

Area of Science:

  • Biomolecular research
  • Biotechnology
  • Nanotechnology

Background:

  • Protein complex and aggregate analysis is crucial for research and development.
  • Existing detection techniques are limited in convenience and scope.
  • Gold nanoparticles offer unique optical properties for enhanced detection.

Purpose of the Study:

  • To develop a novel, label-free assay for detecting protein aggregates.
  • To enhance the sensitivity and convenience of protein complex analysis.
  • To investigate the aggregation behavior of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH).

Main Methods:

  • Development of a nanoparticle aggregation immunoassay (NanoDLSay).
  • Utilized gold nanoparticle immunoprobes coupled with dynamic light scattering (DLS).

More Related Videos

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

Related Experiment Videos

Last Updated: Jun 12, 2026

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
06:49

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Published on: December 17, 2021

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

  • Demonstrated the method using Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a model protein.

    • Main Results:

    • NanoDLSay provides a sensitive and label-free method for protein aggregate detection.
    • GAPDH was found to aggregate readily in specific buffer conditions (10-25 microg/ml).
    • Gold nanoparticles significantly boosted the sensitivity of DLS for aggregate detection.

    Conclusions:

    • NanoDLSay is a convenient and sensitive tool for studying protein complexes and aggregates.
    • The assay eliminates the need for fluorescent labeling of protein targets.
    • This method holds promise for advancing biomolecular research and biopharmaceutical development.