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Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
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Related Experiment Video

Updated: May 15, 2025

Isolation and Characterization of RNA-Containing Exosomes
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Rapid Isolation and Characterization of Exosomes through a Single-Step, Label-Free Protein Biomarker Analysis.

Hannah Ambrosius1,2, Manjusha Vaidya3, Fnu Joshua1,2

  • 1Department of Chemistry, University of Central Florida, Physical Sciences Bld, 4111 Libra Dr #255, Orlando, Florida 32816, United States.

ACS Applied Bio Materials
|April 9, 2025
PubMed
Summary

This study introduces a rapid, label-free method to isolate and analyze exosome biomarkers using centrifugation-filtration-concentration (CFC) and dynamic light scattering. This technique enables quick detection of disease-specific exosomes for diagnostic applications.

Keywords:
diagnostic toolsdynamic light scatteringexosome isolationlabel-free biomarker detectionprotein biomarkers

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Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
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An Innovative Method for Exosome Quantification and Size Measurement
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An Innovative Method for Exosome Quantification and Size Measurement

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Related Experiment Videos

Last Updated: May 15, 2025

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

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Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
09:22

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

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An Innovative Method for Exosome Quantification and Size Measurement
11:38

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Biomarker Discovery

Background:

  • Exosomes are extracellular vesicles reflecting cell origin, valuable as disease biomarkers.
  • Current exosome isolation and analysis methods can be time-consuming and complex.
  • Sensitive and specific detection of exosome biomarkers is crucial for early disease diagnosis.

Purpose of the Study:

  • To develop a rapid, single-step, label-free protocol for exosome isolation and protein biomarker analysis.
  • To demonstrate the utility of the protocol for characterizing exosomes from different cell types.
  • To establish a convenient platform for developing rapid diagnostic tests based on exosome biomarkers.

Main Methods:

  • Exosomes were isolated and enriched 50-fold using a centrifugation-filtration-concentration (CFC) method.
  • Label-free protein biomarker analysis was performed by adding unconjugated antibodies to isolated exosomes.
  • Exosome aggregation and subsequent average particle size increase were detected using dynamic light scattering.

Main Results:

  • The CFC method successfully isolated exosomes from HEK293, HEK-GFP, and GBM/NSC CD133+ cells.
  • Antibody binding to specific exosome surface proteins induced a detectable increase in particle size (25-148 nm).
  • The size increase correlated with the presence of target biomarkers, while negative controls showed minimal changes.

Conclusions:

  • The developed protocol offers a rapid and convenient method for exosome isolation and biomarker detection.
  • This label-free approach facilitates the design of diagnostic tests targeting disease-specific exosomes.
  • The technique holds promise for advancing early disease detection through exosome analysis.