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A cyclic enzymatic amplification method for sensitive and selective detection of nucleic acids

Liang Cui1, Guoliang Ke, Chunming Wang

  • 1State Key Laboratory for Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

The Analyst
|June 22, 2010
PubMed
Summary

We developed a Cyclic Enzymatic Amplification Method (CEAM) for sensitive DNA detection. This novel approach uses Exonuclease III and probes to achieve picomolar sensitivity in under 20 minutes.

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