Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design of a Stable Cyclic Peptide Analgesic Derived from Sunflower Seeds that Targets the κ-Opioid Receptor for the Treatment of Chronic Abdominal Pain.

Journal of medicinal chemistry·2021
Same author

An Integrated Molecular Grafting Approach for the Design of Keap1-Targeted Peptide Inhibitors.

ACS chemical biology·2021
Same author

Hormone-like conopeptides - new tools for pharmaceutical design.

RSC medicinal chemistry·2021
Same author

<sup>13</sup> C NMR studies of insulin. Part I-Spectral assignments.

Magnetic resonance in chemistry : MRC·2021
Same author

Engineered Conotoxin Differentially Blocks and Discriminates Rat and Human α7 Nicotinic Acetylcholine Receptors.

Journal of medicinal chemistry·2021
Same author

Tuning the Anti-Angiogenic Effect of the P15 Peptide Using Cyclic Trypsin Inhibitor Scaffolds.

ACS chemical biology·2021
Same journal

Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications.

Biopolymers·2026
Same journal

Synthesis, Characterization, and Antidiabetic Evaluation of Sequence-Modified Liraglutide Analogs in a Drosophila melanogaster Model.

Biopolymers·2026
Same journal

Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.

Biopolymers·2026
Same journal

Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.

Biopolymers·2026
Same journal

Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.

Biopolymers·2026
Same journal

Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.

Biopolymers·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

A new "era" for cyclotide sequencing.

Michelle L Colgrave1, Aaron G Poth, Quentin Kaas

  • 1CSIRO Livestock Industries, 306 Carmody Rd, St Lucia, QLD 4067, Australia. michelle.colgrave@csiro.au

Biopolymers
|June 22, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a new proteomic strategy to rapidly discover cyclotides, a stable class of cyclic peptides. The method accelerates identification of known, modified, and novel cyclotide sequences from plant extracts.

More Related Videos

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

Related Experiment Videos

Last Updated: Jun 12, 2026

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Cyclotides are a large family of macrocyclic peptides with exceptional stability due to their cyclic backbone and knotted disulfide bridges.
  • Their unique structure, while conferring stability, poses significant challenges for efficient sequencing and identification.
  • Current methods for cyclotide sequencing are slow and inefficient, hindered by extraction difficulties, complex derivatization steps, and lack of specialized bioinformatics tools.

Purpose of the Study:

  • To develop and validate a rapid proteomic strategy for the discovery of cyclotide sequences.
  • To overcome the limitations of existing cyclotide sequencing methodologies.
  • To accelerate the identification of known, modified, and novel cyclotides from plant sources.

Main Methods:

  • Application of proteomic strategies, including optimized Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) conditions.
  • Development of a custom cyclotide database designed to handle circular permutations.
  • Analysis of fractions from Viola odorata and crude leaf extracts from Oldenlandia affinis and Arabidopsis thaliana.

Main Results:

  • Successfully identified 11 new cyclotide sequences, along with numerous known and modified cyclotides from Viola odorata fractions.
  • Validated the methodology by unambiguously identifying a suite of cyclotides in Oldenlandia affinis crude leaf extract.
  • Demonstrated a significant acceleration in the discovery of cyclotide sequences compared to traditional methods.

Conclusions:

  • The developed proteomic strategy significantly fast-tracks cyclotide discovery, marking a new era in sequencing these complex molecules.
  • Optimized LC-MS/MS conditions and a custom database are key advancements for efficient cyclotide identification.
  • This approach provides a powerful tool for exploring the diversity of cyclotides in nature.