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Updated: Jun 12, 2026

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Covering all bases in HIV research: unveiling a hidden world of viral evolution
Bram Vrancken1, Sébastian Lequime, Kristof Theys
1Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium. bram.vrancken@rega.kuleuven.be
Abstract:
The advent of next-generation sequencing technologies has greatly impacted genomic research by providing a means for increasing the amount of sequence information in a cost effective fashion. Among the major next-generation sequencing technologies, the Roche 454 platform has been widely adopted in HIV research. We discuss a broad range of applications of the 454 pyrosequencing platform in the HIV field, with a particular emphasis on antiretroviral therapy and virus-host interaction-related research. We also highlight some of the bioinformatics challenges, as well as advantages and potential limitations of this "deep" sequencing tool, and hint at future research applications.
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