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Updated: Jun 12, 2026

Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents
Published on: January 22, 2017
[NADPH-diaphorase-positive structures in the spinal cord and spinal ganglia]
Abstract:
Using the histochemical reaction with the various incubation medium pH, the localization of NADPH-diaphorase was studied in the spinal cord and spinal ganglia of the adult rat. The data obtained indicate, that the application of the incubation medium with pH 7.4 allows to demonstrate the neurons with various NADPH-diaphorase activity, to define not only the density of enzyme distribution, but also to calculate the numbers of NADPH-diaphorase-positive neurons. It was established, that both in the spinal cord and spinal ganglia, NADPH-diaphorase activity was found not in all the neurons: high activity was found in small and medium neurons of the spinal ganglia, while low activity was detected in motoneurons. Other cellular structures of the spinal cord also possessed NADPH-diaphorase activity and were found to be the interneurons.
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