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Updated: Jun 12, 2026
07:34
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
Fourier transform mass spectrometry for metabolome analysis
C Junot1, G Madalinski, J-C Tabet
1Service de Pharmacologie et d'Immuno-analyse, DSV/iBiTec-S, CEA/Saclay, 91191 Gif-sur-Yvette cedex, France. christophe.junot@cea.fr
The Analyst
|June 25, 2010
Summary
Fourier transform mass spectrometry (FT/MS) enhances metabolomics by offering accurate mass measurements and high resolution. This technology improves metabolite profiling, quantification, and structural characterization for deeper biological insights.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Metabolomics
Background:
- The metabolome comprises diverse molecules requiring advanced analytical techniques for comprehensive analysis.
- Atmospheric pressure ionization Fourier transform mass spectrometry (FT/MS) offers high accuracy and resolution, making it suitable for metabolomics.
Purpose of the Study:
- To evaluate the advancements in metabolomics offered by various FT/MS instruments.
- To assess the capability of FT/MS platforms in addressing key metabolomic requirements: profiling, quantification, and structural characterization.
Main Methods:
- Utilizing different types of FT/MS instruments for metabolomic analysis.
- Evaluating data pre-processing specificities and the impact of accurate mass measurements.
Main Results:
- FT/MS instruments provide significant improvements for global metabolite profiling.
- Accurate mass measurements from FT/MS enhance absolute quantification and structural elucidation.
- The technology aids in biological interpretation and the identification of metabolic networks.
Conclusions:
- FT/MS is a powerful technology for comprehensive metabolomics, supporting diverse analytical needs.
- The precise data generated by FT/MS is crucial for advancing biological interpretation and metabolic network analysis.
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