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Updated: Jun 11, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
Published on: July 5, 2024
Improved DNA electrophoresis in conditions favoring polyborates and lewis acid complexation
Hari Singhal1, Yunzhao R Ren, Scott E Kern
1Department of Biomedical Engineering, The Johns Hopkins University and the Sidney Kimmel Comprehensive Center at Johns Hopkins, Baltimore, Maryland, United States of America.
Abstract:
Spatial compression among the longer DNA fragments occurs during DNA electrophoresis in agarose and non-agarose gels when using certain ions in the conductive buffer, impairing the range of fragment sizes resolved well in a single gel. Substitutions using various polyhydroxyl anions supported the underlying phenomenon as the complexation of Lewis acids to DNA. We saw significant improvements using conditions (lithium borate 10 mM cations, pH 6.5) favoring the formation of borate polyanions and having lower conductance and Joule heating, delayed electrolyte exhaustion, faster electrophoretic run-speed, and sharper separation of DNA bands from 100 bp to 12 kb in a single run.
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