Related Experiment Video
Updated: Jun 11, 2026
14:23
Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
Published on: August 27, 2012
Novel invadopodia components revealed by differential proteomic analysis.
Francesca Attanasio1, Giusi Caldieri, Giada Giacchetti
1Tumor Cell Invasion Laboratory, Consorzio Mario Negri Sud, S. Maria Imbaro, Chieti 66030, Italy.
European Journal of Cell Biology
|July 9, 2010
Summary
Researchers identified 58 new proteins in invadopodia, crucial for cancer cell invasion. This proteomic study enhances understanding of cancer cell metastasis and extracellular matrix degradation.
Area of Science:
- Cell Biology
- Proteomics
- Cancer Research
Background:
- Highly invasive cancer cells form invadopodia to degrade the extracellular matrix.
- The molecular composition of invadopodia is not fully understood, hindering research into cancer cell invasion.
Purpose of the Study:
- To accelerate the discovery of invadopodia components using a proteomics approach.
- To identify novel proteins involved in cancer cell invasion and metastasis.
Main Methods:
- Utilized Difference Gel Electrophoresis (DIGE) to compare invadopodia-enriched fractions with other cellular fractions.
- Performed peptide mass fingerprinting to identify proteins from selected spots.
- Validated identified proteins using immunoblotting and immunofluorescence localization.
Main Results:
- Successfully identified 58 proteins, including those involved in metabolism, protein synthesis/degradation, cytoskeleton, and membrane functions.
- Demonstrated enrichment of known invadopodia-associated proteins during fractionation.
- Validated several newly identified proteins through biochemical and imaging techniques.
Conclusions:
- The study identified novel components of invadopodia, expanding the knowledge of their molecular makeup.
- These findings provide new targets for studying the invasive behavior of cancer cells.
- Opens new avenues for understanding and potentially targeting cancer cell metastasis.
More Related Videos
Related Concept Videos
01:35Cancer Cell Migration through Invadopodia
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...
01:39Mechanism of Filopodia Formation
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
01:32Pinching-off of Coated Vesicles
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
01:31Mechanism of Lamellipodia Formation
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
01:28Types of Membrane Protrusions
The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
The microvilli, an example of stable protrusions, are finger-like projections with a...
The microvilli, an example of stable protrusions, are finger-like projections with a...