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iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Updated: Jun 11, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A Cell Programmable Assay (CPA) chip.

Jongil Ju¹, Jay Warrick, David J Beebe

¹Department of Biomedical Engineering & Wisconsin Institutes for Medical Research, University of Wisconsin-Madison, Madison, WI 53705, USA.

|July 9, 2010

Summary

This summary is machine-generated.

Two Cell Programmable Assay (CPA) chips simplify cell culture and staining. These microfluidic devices automate common lab protocols, offering flexibility for various cell biology applications.

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Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Traditional cell culture and staining protocols are often time-consuming and labor-intensive.
Microfluidic devices offer potential for automating and simplifying biological assays.

Purpose of the Study:

To describe and evaluate two novel Cell Programmable Assay (CPA) chips for automated cell culture and staining.
To introduce a simplified equation for the design and modification of CPA chips.

Main Methods:

Development of single timer channel (sCPA) and dual timer channel (dCPA) CPA chips.
Utilizing passive pumping for cell culture and autonomous staining (Hoechst and DAPI).
Evaluation of CPA chips using HEK 293 cells.

Main Results:

Successful demonstration of cell culture and autonomous staining using both sCPA and dCPA chips.
The dCPA chip automated a 3-step staining process.
A simplified equation was derived for CPA chip tuning based on microchannel dimensions and reagent volumes.

Conclusions:

CPA chips provide a simplified and automated approach to common cell culture and staining protocols.
The sCPA and dCPA chips are adaptable for a wide range of cell-based assays.
The provided equation facilitates the customization of CPA chips for diverse experimental needs.