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Related Experiment Video

Updated: Jun 11, 2026

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

Tissue-engineered vascular grafts: does cell seeding matter?

Tamar L Mirensky1, Narutoshi Hibino, Rajendra F Sawh-Martinez

  • 1Yale-New Haven Hospital, New Haven, CT 06510, USA; Yale University School of Medicine, New Haven, CT 06510, USA. tamar.mirensky@yale.edu

Journal of Pediatric Surgery
|July 13, 2010
PubMed
Summary

Monocytes are crucial for the patency and diameter of tissue-engineered vascular grafts (TEVGs) in congenital heart defect repair. Seeding grafts with monocytes promotes neovessel formation and successful integration.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Vascular Biology

Background:

  • Tissue-engineered vascular grafts (TEVGs) offer growth potential for congenital heart defect repair.
  • Mechanisms of neovessel formation in TEVGs are not fully understood.

Purpose of the Study:

  • To define the role of seeded monocytes from bone marrow mononuclear cells (BM-MNCs) in neovessel formation within TEVGs.

Main Methods:

  • Biodegradable tubular scaffolds were seeded with BM-MNCs, BM-MNCs without monocytes, or only monocytes.
  • Grafts were implanted in mice and evaluated using advanced imaging and histological techniques.

Main Results:

  • All TEVGs remained patent without stenosis or aneurysm.
  • Monocyte-seeded grafts showed significantly larger diameters at 6 months compared to those without monocytes.

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Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique

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Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
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Related Experiment Videos

Last Updated: Jun 11, 2026

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique
07:13

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique

Published on: May 2, 2025

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
07:49

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

  • Neovessels exhibited mature structures with endothelial lining, smooth muscle cells, and collagen.

    • Conclusions:

    • Monocytes play a critical role in maintaining TEVG patency and promoting neovessel maturation.
    • Incorporating monocytes into TEVG design is essential for enhancing graft success in congenital heart defect repair.