Related Experiment Video
Updated: Jun 11, 2026
Surgical Perfusion and Isolation of the Porcine Pancreas for Islet Isolation
Published on: June 7, 2024
Preoxygenation of different preservation solutions for porcine pancreas preservation
F Hackl1, P Stiegler, V Stadlbauer
1Department of Surgery, Division of Transplantation Surgery Medical University of Gray, Austria.
Introduction:
Organ preservation quality impacts porcine islet cell isolation and transplantation success. Among several preservation methods, the two-layer method is promising, but technically demanding and fails to deliver sufficient oxygen. The use of hyperbaric oxygenation may be an easier, more effective method to supply high partial pressure of oxygen (pO(2)) for organ storage. Therefore, the aim of this study was to test the capability of preoxygenation of various preservation solutions with HBO to maintain high pO(2) levels.
Methods:
University of Wisconsin (UW), Custodiol, Perfadex, or Celsior solutions were preoxygenated in a pressure chamber. NaCl served as the control. pO(2) levels were measured at defined times. The oxygen storage capability was evaluated by leaving the storage bottles open for 2 minutes.
Results:
It was feasible to preoxygenate preservation solutions. The best solution to maintain high pO(2) tensions was Perfadex, followed by Celsior, and UW.
Discussion:
The greater the amount of oxygen in the preservation solution, the more oxygen can be delivered to the preserved pancreas. Further studies on the influence of preoxygenated preservation solutions on the porcine pancreas are warranted to improve organ quality, porcine islet cell isolation, and transplantation success.
