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Controlled delivery systems for proteins based on poly(lactic/glycolic acid) microspheres.

S Cohen1, T Yoshioka, M Lucarelli

  • 1Department of Chemical Engineering, MIT, Cambridge, Massachusetts 02139.

Pharmaceutical Research
|June 1, 1991
PubMed
Summary

Poly(lactic/glycolic acid) (PLGA) microspheres effectively deliver proteins long-term. Encapsulation protects enzymes from degradation, with release profiles tunable by adjusting preparation methods.

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery Systems

Background:

  • High molecular weight, water-soluble proteins require stable delivery systems.
  • Poly(lactic/glycolic acid) (PLGA) is a biodegradable polymer often used in drug delivery.

Purpose of the Study:

  • To investigate the use of PLGA microspheres for long-term delivery of proteins.
  • To evaluate the stability and release characteristics of proteins encapsulated in PLGA.

Main Methods:

  • PLGA microspheres prepared using a modified double emulsion solvent evaporation method.
  • Encapsulation of fluorescein isothiocyanate-labeled bovine serum albumin and horseradish peroxidase.
  • Stability, in vitro release, and degradation studies were performed.

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Main Results:

  • Microspheres were spherical (55-95 microns) with >90% protein encapsulation efficiency.
  • Encapsulated enzymes showed significantly reduced activity loss compared to free enzymes.
  • Tunable release profiles (near-constant or biphasic) were achieved by modifying preparation parameters.

Conclusions:

  • PLGA microspheres provide a protective environment for protein stability.
  • Protein release is primarily mediated by PLGA matrix erosion.
  • The preparation method allows for controlled protein release kinetics for long-term delivery applications.