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Related Experiment Video

Updated: Jun 10, 2026

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

Imaging lipids in living cells.

Carsten Schultz, Anne B Neef, Theodorus W Gadella

    Cold Spring Harbor Protocols
    |July 22, 2010
    PubMed
    Summary
    This summary is machine-generated.

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    Imaging lipids in cells is challenging due to their small size. This study introduces new methods for fluorescently tagging lipids, enabling visualization of their location and dynamics within cells.

    Area of Science:

    • Cell Biology
    • Biochemistry

    Background:

    • Investigating lipids in living cells remains an underdeveloped area in cell biology.
    • Fractionating cellular membranes to determine lipid location and dynamics is difficult due to membrane contact.
    • Current methods for molecular imaging, like fluorescent proteins, are unsuitable for small molecules such as lipids.

    Purpose of the Study:

    • To address the challenge of visualizing lipids within living cells.
    • To develop and describe novel techniques for imaging lipid location, dynamics, and metabolism.
    • To overcome limitations of traditional fluorescent tagging for small molecules.

    Main Methods:

    • Utilizing fluorescently labeled proteins that specifically recognize lipids.
    • Employing chemical methods to introduce fluorescent tags into lipid derivatives within cells.

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    Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
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    Published on: January 31, 2025

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    Last Updated: Jun 10, 2026

    Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
    10:59

    Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

    Published on: May 28, 2021

    Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
    07:20

    Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

    Published on: January 31, 2025

  • Applying these techniques to image lipids in both fixed and living cells.

    • Main Results:

    • Successful development of procedures for imaging lipids using fluorescently tagged lipid-binding domains.
    • Demonstration of effective lipid derivative labeling for cellular imaging.
    • Enabled visualization of lipid location and dynamics, overcoming previous limitations.

    Conclusions:

    • The described methods provide effective solutions for imaging lipids in cellular environments.
    • These techniques advance the study of lipid localization, dynamics, and metabolism.
    • Opens new avenues for understanding lipid roles in cell biology.