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Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
Published on: September 24, 2021
Bioenergetics and cell death.
Yulia Kushnareva1, Donald D Newmeyer
1La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA.
Annals of the New York Academy of Sciences
|July 24, 2010
Summary
Mitochondrial outer membrane permeabilization (MOMP) disrupts cell energy production, triggering irreversible cell death. This bioenergetic collapse can lead to necrosis, even without caspase activation, highlighting MOMP
Area of Science:
- Cellular biology
- Mitochondrial function
- Cell death pathways
Background:
- Mitochondrial bioenergetic function is crucial for both cell survival and programmed cell death (apoptosis).
- Apoptosis execution involves energy-dependent steps like kinase signaling and caspase activation.
- Bioenergetic collapse can redirect cells towards necrosis.
Purpose of the Study:
- To discuss the mechanisms by which mitochondrial outer membrane permeabilization (MOMP) impacts cellular bioenergetics.
- To explore how MOMP influences different cell death paradigms.
Main Methods:
- Review and discussion of existing literature on MOMP and bioenergetics.
- Analysis of mechanisms linking MOMP to bioenergetic decline beyond cytochrome c release.
Main Results:
- MOMP is a critical event in apoptosis execution and is causally linked to decreased bioenergetic function.
- MOMP-induced bioenergetic deficiency is often irreversible and can commit cells to die, irrespective of caspase activity.
- Mechanisms of bioenergetic impact by MOMP differ across various cell death contexts.
Conclusions:
- Mitochondrial outer membrane permeabilization significantly impairs cellular bioenergetics, contributing to cell death.
- The bioenergetic consequences of MOMP are a key factor in determining cell fate, potentially overriding apoptotic signaling.
- Understanding MOMP's impact on bioenergetics is essential for deciphering cell death mechanisms.
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