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Updated: Jun 10, 2026
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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
Published on: March 9, 2017
Inkjet-printed paperfluidic immuno-chemical sensing device
Koji Abe1, Kaori Kotera, Koji Suzuki
1Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.
Analytical and Bioanalytical Chemistry
|July 24, 2010
Summary
This study introduces a novel inkjet-printed paperfluidic device for rapid, low-cost immunosensing. The single-piece filter paper design simplifies fabrication and enables sensitive detection of human IgG, offering potential for portable diagnostics.
Area of Science:
- Microfluidics
- Biosensing
- Immunochromatography
Background:
- Conventional immunochromatographic strips require complex assembly with multiple components.
- Existing methods are often time-consuming and costly for widespread diagnostic applications.
Purpose of the Study:
- To develop a simplified, low-cost fabrication method for lateral flow immunochromatographic devices.
- To demonstrate a paperfluidic immunosensing device capable of sensitive and rapid analyte detection.
- To explore the potential for multianalyte detection using a single paper substrate.
Main Methods:
- Inkjet printing of microfluidic channels and immunosensing inks onto a single piece of filter paper.
- Physical adsorption for immobilizing antibodies on test and control lines.
- Sandwich immunoreaction for human IgG detection and colorimetric analysis.
Main Results:
- Achieved sensitive detection of human IgG down to 10 μg/l within 20 minutes.
- Demonstrated a simplified fabrication process applicable to cellulose surfaces.
- Successfully fabricated a multianalyte device integrating both immunoassay and chemical sensing.
Conclusions:
- The inkjet-printed paperfluidic device offers a cost-effective and time-efficient alternative to traditional immunochromatographic strips.
- This technology is suitable for portable, disposable diagnostic tools in medical, environmental, and food safety applications.
- The approach holds promise for use in remote settings and resource-limited regions.
