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Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

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Published on: April 11, 2013

A hard-soft microfluidic-based biosensor flow cell for SPR imaging application.

Changchun Liu¹, Dafu Cui, Hui Li

¹State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China. liucc263@yahoo.com.cn

Biosensors & Bioelectronics

|July 27, 2010

Summary

This summary is machine-generated.

Researchers developed a novel hard-soft biosensor flow cell using a SiO(2) thin film to bond polydimethylsiloxane (PDMS) to PMMA. This robust microfluidic device enhances biosensing applications, including surface plasmon resonance imaging assays.

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

Materials Science
Biotechnology
Chemical Engineering

Background:

Microfluidic biosensor flow cells require both soft interfaces for sealing and hard interfaces for tubing.
These properties are mutually exclusive in single materials, necessitating innovative solutions.
Existing designs face limitations in achieving both robust sealing and reliable connectivity.

Purpose of the Study:

To develop a novel hard-soft microfluidic biosensor flow cell.
To utilize a SiO(2) thin film as an intermediate layer for bonding PDMS to a plastic substrate.
To create a compact and robust flow cell for multi-array immunoassay applications, particularly with surface plasmon resonance (SPR) imaging.

Main Methods:

Fabrication of a rigid poly(methyl methacrylate) (PMMA) base using computer numerically controlled (CNC) machining.
Deposition of a 200 nm SiO(2) thin film via plasma-enhanced chemical vapor deposition (PECVD).
Irreversible adhesion of a soft polydimethylsiloxane (PDMS) microfluidic layer to the SiO(2)-coated PMMA base.

Main Results:

Successful creation of a hard-soft biosensor flow cell combining soft sealing and hard connectivity.
The device maintained the advantages of PDMS flow cells (soft interface, ease of fabrication, low cost) while adding a robust interface for tubing.
The flow cell operated reliably up to 185 kPa in aqueous environments.
Demonstrated application in SPR imaging for real-time immunoglobulin G (IgG) interaction monitoring.
Achieved high sensitivity in detecting sulfamethoxazole (SMOZ) and sulfamethazine (SMZ) at 3.5 and 0.6 ng/mL, respectively.

Conclusions:

The novel hard-soft microfluidic device offers a significant advancement for biosensor flow cells.
This design overcomes material limitations, providing a compact, robust, and user-friendly solution.
The developed flow cell is versatile and applicable to various other biosensing platforms and assays.