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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Pharmacogenomics: Identification of New Drug Targets01:29

Pharmacogenomics: Identification of New Drug Targets

Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Drug Discovery: Overview01:26

Drug Discovery: Overview

Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

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Related Experiment Video

Updated: Jun 10, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Drug discovery and mutant p53.

Magda M Maslon1, Ted R Hupp

  • 1University of Edinburgh, Institute of Genetics and Molecular Medicine, Cell Signalling Unit, Cancer Research UK p53 Signal Transduction Group, Edinburgh EH4 2XR, UK.

Trends in Cell Biology
|July 27, 2010
PubMed
Summary

Targeting mutant p53 pathways offers new cancer therapies. Advances in understanding mutant p53's pro-oncogenic functions and developing reactivation strategies are paving the way for novel drug discovery.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biophysics

Background:

  • Missense mutations in the p53 tumor suppressor gene are frequent in human cancers.
  • These mutations inactivate p53's tumor-suppressive functions but can stabilize a pro-oncogenic mutant p53 protein.
  • Understanding mutant p53's role is crucial for developing targeted cancer therapies.

Purpose of the Study:

  • To review recent multidisciplinary advances in targeting the mutant p53 pathway.
  • To highlight novel strategies for refolding and reactivating mutant p53.
  • To explore the pro-oncogenic signaling and interactome of mutant p53.

Main Methods:

  • Biophysical modeling to understand p53 inactivation mechanisms.
  • Analysis of mutant p53 protein stability and degradation pathways (e.g., MDM2-mediated).

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Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay

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Last Updated: Jun 10, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
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Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
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Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

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Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay

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  • Mapping the mutant p53 interactome to identify oncogenic signaling networks.

    • Main Results:

    • Biophysical models explain p53 mutation-induced inactivation.
    • Mutant p53 can evade degradation, contributing to its oncogenic potential.
    • The mutant p53 interactome reveals its role in diverse oncogenic and pro-metastatic signaling.

    Conclusions:

    • Therapeutic targeting of mutant p53 pathways shows significant promise.
    • Advances in understanding mutant p53's function facilitate drug discovery.
    • Focus on protein-protein interactions and conformational control is key for targeting mutant p53.