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Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

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Tissue-specific expression of TIPE2 provides insights into its function.

Guizhong Zhang1, Chunyan Hao, Yunwei Lou

  • 1Institute of Immunology, School of Medicine, Shandong University, Ji'nan, PR China.

Molecular Immunology
|July 29, 2010
PubMed
Summary

Tumor necrosis factor-alpha-induced protein-8 like-2 (TIPE2) is a negative regulator of immunity. This study identified TIPE2 protein expression in specific immune cells and tissues, revealing its role in cellular immunity.

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Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Published on: April 13, 2022

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Tumor necrosis factor-alpha-induced protein-8 like-2 (TIPE2) is a newly identified negative regulator of innate and cellular immunity.
  • TIPE2 deficiency in mice results in fetal inflammatory diseases, and its downregulation in humans is linked to systemic autoimmunity.
  • Previous studies were limited by the lack of a specific antibody to TIPE2, hindering the determination of its protein expression patterns.

Purpose of the Study:

  • To generate a highly specific antibody for TIPE2.
  • To investigate the tissue and cellular distribution of TIPE2 protein expression in mice.
  • To elucidate the functional implications of TIPE2's tissue-specific expression.

Main Methods:

  • Generation of a specific anti-TIPE2 antibody.
  • Immunohistochemistry to detect TIPE2 protein in murine tissues.
  • RT-PCR to assess TIPE2 mRNA expression.

Main Results:

  • TIPE2 is a cytoplasmic protein predominantly expressed in lymphoid tissues, particularly in T cells.
  • B cells and B cell zones of lymphoid organs showed no TIPE2 expression.
  • Detectable TIPE2 protein and mRNA were found in some non-lymphoid tissues, including endocrine tissues and skeletal muscle.
  • High TIPE2 levels were observed in monocyte/macrophage cell lines and ovarian cancer cells, but not in most human carcinoma cell lines.

Conclusions:

  • TIPE2 exhibits tissue-specific expression patterns in both lymphoid and non-lymphoid compartments.
  • The distinct expression profile of TIPE2 may explain its role in enhancing cellular immunity while having a selective defect in humoral immunity.
  • These findings provide insights into the specific functions of TIPE2 in immune regulation and disease.