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Updated: Jun 10, 2026
Transient Transduction of the Strobilated Forms of Echinococcus granulosus
Published on: September 16, 2022
[Prokaryotic expression and immunologic identification of P-29 gene from Echinococcus granulosus hydatid cyst]
Jie Li1, Zhi-gang Wang, Xi-yan Hao
1College of Life Science, Inner Mongolia University, Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China.
Objective:
To clone and express P-29 gene of Echinococcus granulosus, and analyze its immunoreactivity.
Methods:
Total RNA was extracted from the hydatid cyst of E. granulosus and its P-29 gene was amplified by RT-PCR. The PCR product was cloned into pET44a(+) vector. The recombinant plasmid was transformed into E. coli BL21 (DE3) and followed by expression of the protein induced by IPTG. The protein was purified, and tested by SDS-PAGE. Its immunoreactivity was examined by Western blotting.
Results:
The recombinant expression plasmid was identified by PCR, double endonuclease digestion and sequencing. The recombinant Nus-P-29 was about Mr 93 000 with a concentration of 0.78 mg/ml. It was recognized by the sera of cystic echinococcosis patients.
Conclusion:
The P-29 gene has been expressed with adequate immunoreactivity.
