Related Experiment Video
Updated: Jun 10, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Optimization of immobilization for selective oxidation of benzyl alcohol by Gluconobacter oxydans using response
Jian Wu1, Jia-Le Wang, Min-Hua Li
1New World Institute of Biotechnology, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
This study used the Box-Behnken design and response surface methodology to optimize immobilization of Gluconobacter oxydans in Ca-alginate gel for the production of benzaldehyde in a biphasic system. Immobilization parameters, such as Na-alginate concentration, cell load, and bead diameter, were optimized. The mathematical model developed was validated and proven to be statistically adequate and accurate in predicting the response. For both activity and stability responses, the best results were achieved at alginate concentration of 2.55% (w/v), cell load of 49.26 mg/ml, and 2.2 mm bead diameter. Under these conditions, retention activity of 87.6% could be attained for the immobilized cell. In addition, the oxidative activity of immobilized cells was retained at 53.2% compared with that of free cells after 10 repeated batch reactions, while only 15.7% of activity remained in free cells.
Related Concept Videos
Reactions of Aldehydes and Ketones: Baeyer–Villiger Oxidation
The carbonyl center is activated by...
Methods of Medium Optimization
Production of Alcohol
Oxidation of Alcohols
The process of oxidation in a chemical reaction is observed in any of the three forms:
Hydroboration-Oxidation of Alkenes
Preparation of Alcohols via Addition Reactions
The acid-catalyzed addition of water to the double bond of alkenes is a large-scale industrial method used to synthesize low-molecular-weight alcohols. An acidic atmosphere is required to allow the hydrogen in the water molecule to act as an electrophile and attack the double bond in an alkene. The addition of a proton to the double bond creates a carbocation intermediate. The proton preferentially bonds to the less substituted end of the double bond to create a more stable carbocation...

