Related Experiment Video
Updated: Jun 10, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
A safe and practical procedure for global deprotection of oligoribonucleotides
Daniel Zewge1, Francis Gosselin, Rick Sidler
1Department of Process Research, Merck Research Laboratories, P.O. Box 2000, Rahway, New Jersey 07065, USA. daniel_zewge@merck.com
Abstract:
We report a practical global deprotection of RNA 2'-O-tert-butyldimethylsilyl (TBS) ethers using commercially available aqueous NH(4)F. The procedure is applicable to both 96-well plate format and large-scale production of RNA. This improved procedure provides a safe, mild, and cost-effective alternative to highly hazardous Et(3)N x 3 HF, a reagent commonly used in the routine synthesis of RNA.
More Related Videos
11:37Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
Published on: July 28, 2017
06:58Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
Published on: October 7, 2021