Development of a sensitive and selective Riboflavin sensor based on carbon ionic liquid electrode.

Area of Science:

• Electrochemistry
• Analytical Chemistry
• Materials Science

Background:

• Riboflavin (Vitamin B2) is essential for human health.
• Accurate quantification of Riboflavin is crucial for food, pharmaceutical, and nutritional analysis.
• Electrochemical methods offer sensitive and selective detection pathways.

Purpose of the Study:

• To investigate the electrochemical behavior of Riboflavin adsorbed on a carbon ionic liquid electrode (CILE).
• To develop and validate a sensitive electrochemical method for Riboflavin determination.
• To assess the applicability of the developed method for real-world sample analysis.

Main Methods:

• Cyclic voltammetry was employed to study Riboflavin adsorption and film formation on the CILE.
• Surface coverage and electrochemical parameters (electron transfer coefficient, rate constant) were determined.
• Differential pulse voltammetry was utilized for quantitative Riboflavin analysis.

Main Results:

• A stable Riboflavin film formed on the CILE with a surface coverage up to 3.3x10(-9) mol cm(-2).
• Electrochemical parameters indicated efficient electron transfer across the modified electrode.
• The method achieved two linear working ranges (0.8-110 nM and 0.11-1.0 microM) with a low detection limit of 0.1 nM.
• High precision (RSD 4.7%) and minimal interference from other vitamins were observed.

Conclusions:

• The CILE is a suitable platform for sensitive electrochemical detection of Riboflavin.
• The developed method demonstrates excellent performance characteristics for Riboflavin quantification.
• The method is applicable for determining Riboflavin in pharmaceutical products, nutritional supplements, and beverages.