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Updated: Jun 10, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Published on: August 5, 2009
Imaging of vibrating objects using speckle subtraction
Thomas R Moore1, Ashley E Cannaday, Sarah A Zietlow
1Department of Physics, Rollins College, Winter Park, Florida 32789, USA. tmoore@rollins.edu
Abstract:
A simple method for imaging vibrational motion is proposed. The process consists of capturing two speckled images of a region illuminated by coherent radiation. One of the images is captured before the onset of motion and the other during motion. If the mean speckle intensity is below the threshold for detection or above the saturation intensity of the detector, subtracting the two images produces a high contrast image of the moving region. A theory is shown to agree well with experimental data.
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