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Related Experiment Video

Updated: Jun 10, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Recipient block TMA technique.

Martina Mirlacher1, Ronald Simon

  • 1Institute of Pathology, University Medical Center Hamburg Eppendorf, Hamburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2010
PubMed
Summary
This summary is machine-generated.

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High-throughput screening identifies potential disease genes. Tissue Microarray (TMA) analysis of multiple tissues prioritizes these candidates, offering an affordable and accessible method for gene expression studies.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • High-throughput screening identifies numerous genes implicated in cancer and other diseases.
  • Prioritizing candidate genes requires analyzing their expression across diverse tissue types.
  • Tissue Microarray (TMA) is a valuable tool for high-throughput tissue analysis.

Purpose of the Study:

  • To describe the classical recipient block technology for Tissue Microarray (TMA) construction.
  • To highlight TMA as a method for prioritizing candidate genes identified through screening.
  • To emphasize the accessibility and affordability of TMA for gene expression studies.

Main Methods:

  • Utilizes recipient block technology for TMA construction.
  • Involves punching minute cylindrical tissue samples (0.6 mm diameter) from donor blocks.

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Related Experiment Videos

Last Updated: Jun 10, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
11:34

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

  • Transfers tissue cores into recipient paraffin blocks for analysis.

    • Main Results:

    • Enables analysis of up to 1,000 different tissues within a single TMA block.
    • TMA equipment is affordable and user-friendly.
    • Requires basic histology skills for implementation.

    Conclusions:

    • TMA is an established method for high-throughput tissue analysis.
    • Recipient block technology provides a practical approach to TMA construction.
    • TMA facilitates efficient prioritization of candidate genes for disease research.