Jove
Visualize
Contact Us

Related Experiment Videos

Tissue artefacts caused by sponge in embedding cassettes.

J J Kepes1, O Oswald

  • 1University of Kansas College of Health Sciences and Hospital, Kansas City.

The American Journal of Surgical Pathology
|August 1, 1991
PubMed
Summary

Avoid using sponges in tissue processing cassettes, as they can imprint on tissues. Substitute with tissue or lens paper to prevent the loss of small tissue fragments during processing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytomegalovirus infections in allogeneic stem cell recipients after reduced-intensity or myeloablative conditioning assessed by quantitative PCR and pp65-antigenemia.

Bone marrow transplantation·2003
Same author

Case report: microcystic meningioma in a 58-year-old man with multicystic meningioangiomatosis.

Neuropathology and applied neurobiology·2003
Same author

Gliosarcoma with areas of primitive neuroepithelial differentiation and extracranial metastasis.

Clinical neuropathology·2002
Same author

Spectrum of clinical and histopathologic responses to intracranial electrodes: from multifocal aseptic meningitis to multifocal hypersensitivity-type meningovasculitis.

Epilepsia·2001
Same author

Comparison of cyclin A and MIB-1 expression in astrocytic tumors using image-based cell analysis system.

Applied immunohistochemistry & molecular morphology : AIMM·2001
Same author

Supratentorial giant cell ependymoma.

Clinical neuropathology·2001
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Histotechnology
  • Pathology

Background:

  • Embedding cassettes are crucial for tissue processing in histology.
  • The use of sponges within these cassettes has been a practice, but poses risks.

Purpose of the Study:

  • To evaluate the impact of sponges in tissue processing cassettes.
  • To recommend alternative materials for improved tissue preservation.

Main Methods:

  • Comparative analysis of tissue processing with and without sponges.
  • Evaluation of tissue integrity and fragmentation when using sponges versus alternative materials.

Main Results:

  • Sponges cause disruptive imprints on processed tissues.
  • Small tissue fragments can be lost through cassette perforations when sponges are used.

Related Experiment Videos

Conclusions:

  • Sponges should not be used in tissue processing cassettes.
  • Tissue or lens paper are recommended substitutes to prevent tissue damage and loss.