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Related Experiment Video

Updated: Jun 10, 2026

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device
08:28

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Published on: July 18, 2025

FORMING SELF-ASSEMBLED CELL ARRAYS AND MEASURING THE OXYGEN CONSUMPTION RATE OF A SINGLE LIVE CELL.

James R Etzkorn1, Sarah C McQuaide, Judy B Anderson

  • 1Electrical Engineering, University of Washington, Seattle, WA, USA.

Digest of Technical Papers. International Conference on Solid-State Sensors, Actuators, and Microsystems
|August 10, 2010
PubMed
Summary

Researchers developed a novel method using polymer micro-traps to create live single-cell arrays on a chip. This technique enables precise measurement of individual cell oxygen consumption rates, advancing single-cell biology tools.

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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

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Last Updated: Jun 10, 2026

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Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Published on: July 18, 2025

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Published on: April 6, 2022

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity.
  • Existing methods for single-cell manipulation and analysis present challenges in efficiency and precision.
  • Development of novel microfabrication techniques is needed to support advanced single-cell studies.

Purpose of the Study:

  • To develop and validate a method for forming arrays of live single cells on a chip.
  • To assess the biocompatibility of microfabricated polymer structures for cell culture.
  • To establish a technique for measuring the oxygen consumption rate of individual cells.

Main Methods:

  • Utilized SU8 polymer micro-traps for creating single-cell arrays on a chip.
  • Investigated the toxicity of microfabricated structures and the cell culture environment on two cell lines.
  • Employed optical interrogation of molecular oxygen sensors within micro-wells to measure single-cell oxygen consumption rates.
  • Temporarily sealed cells within micro-traps to isolate them for measurement.

Main Results:

  • Achieved a single-cell self-assembly yield of 61%.
  • Demonstrated successful measurement of oxygen consumption rates for isolated live A549 cells.
  • Observed oxygen draw down rates of 0.83, 0.82, and 0.71 fmol/minute for three individual cells.
  • Confirmed the biocompatibility of the micro-trap environment for the studied cell lines.

Conclusions:

  • The developed method effectively forms arrays of live single cells on a chip.
  • The micro-trap system is biocompatible and suitable for studying cellular functions.
  • This technique provides a valuable new tool for quantitative single-cell biology, particularly for metabolic rate measurements.