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Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
The Electrical Double Layer01:30

The Electrical Double Layer

In the region where two bulk phases meet, an intricate electric charge distribution arises due to charge transfer, ion adsorption, molecular orientation, and charge distortion. This complex distribution is commonly referred to as the electrical double layer.When a solid electrode interfaces with ions in an electrolyte solution, the speed of electron transfer dictates the rates of oxidation and reduction. The electrode acquires a charge through the escape of atoms into the solution as cations or...
Electrochemical Systems01:24

Electrochemical Systems

Electrochemical systems provide a fascinating insight into the dynamic interplay of charged species within various phases. One notable example is the interaction between a membrane permeable to K⁺ ions but not to Cl⁻ ions, separating an aqueous KCl solution from pure water. As K⁺ ions diffuse through the membrane, they generate net charges on each phase, leading to a potential difference between them.Similarly, when a piece of Zn is immersed in an aqueous ZnSO₄ solution, the Zn metal, composed...

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Related Experiment Video

Updated: Jun 10, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Interaction between cells in dielectrophoresis and electrorotation experiments.

Miguel Sancho, Genoveva Martínez, Sagrario Muñoz

    Biomicrofluidics
    |August 11, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study models cell interactions in radio frequency (rf) fields using boundary element methods. The research validates the model by examining T lymphocyte pearl chaining and insulinoma cell torques, advancing bioparticle manipulation techniques.

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    Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

    Published on: February 4, 2011

    Area of Science:

    • Biophysics
    • Electrical Engineering
    • Cell Biology

    Background:

    • Microelectrode technologies enable advanced cell and bioparticle manipulation.
    • Theoretical modeling of electrical responses is crucial for these applications.
    • Understanding particle interactions in external fields is key for separation and manipulation.

    Purpose of the Study:

    • To analyze the interaction between cells and radio frequency (rf) fields.
    • To develop and validate a theoretical model for predicting electrical responses of compartmentalized bioparticles.
    • To characterize dielectric properties of cells using numerical simulations.

    Main Methods:

    • Integral formulation based on induced charge densities at particle interfaces.
    • Numerical solution using the boundary element technique (BET).
    • Experimental validation using human T lymphocytes and insulinoma beta-cells.

    Main Results:

    • The model accurately characterizes dielectric properties of bioparticles.
    • Observed the influence of dipolar pearl chaining on T lymphocyte dielectrophoresis.
    • Measured frequency-dependent spin and orbital torques of insulinoma beta-cells in rotating fields.

    Conclusions:

    • The developed theoretical model provides accurate predictions for cell behavior in rf fields.
    • Experimental results validate the model's capability to describe complex particle interactions.
    • This work advances the understanding and manipulation of bioparticles using electrical fields.