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Updated: Jun 10, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
[Detection of molecular epidemiology of Mycobacterium gordonae isolates]
Shiomi Yoshida1, Katsuhiro Suzuki, Tomotada Iwamoto
1Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan. dustin@kch.hosp.go.jp
Objects:
To analyze the molecular epidemiology of Mycobacterium gordonae strains from patients and environments in the hospital.
Subjects:
A total of 46 clinical strains were obtained from patients registered at the NHO Kinki-chuo Chest Medical Center and 3 strains from hospital environments.
Methods:
By using genetic data from the 16S rRNA gene and hsp65PRA, pulsed-field gel electrophoresis (PFGE) assessment of their intraspecies variability and epidemiology was carried out.
Results:
Strains from six patients and environmental cultures exhibited the different genotypes of 16S rRNA gene sequencing and the hsp65PRA type. The PFGE analysis suggested no pseudo-outbreak and showed a polyclonal infection in one patient.
Conclusion:
These findings suggest that we should maintain effective surveillance of environments in the hospital and continuously perform molecular epidemiological investigations for infection control of M. gordonae.
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