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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Selection of lipase-producing microorganisms through submerged fermentation
Luciane Maria Colla1, Andreiza Lazzarotto Primaz, Silvia Benedetti
1Laboratório de Fermentações, Curso de Engenharia de Alimentos, Universidade de Passo Fundo, Passo Fundo, RS, Brazil.
Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|August 27, 2010
Summary
Researchers screened 27 fungi from soil and wastewater for lipase production. Penicillium E-3 showed the highest lipolytic activity, indicating potential for industrial enzyme applications.
Area of Science:
- Biotechnology
- Enzymology
- Microbial Ecology
Background:
- Lipases are crucial enzymes with widespread industrial applications in food, pharmaceuticals, and chemical synthesis.
- Discovering novel microorganisms with enhanced lipase productivity and diverse catalytic activities is an ongoing research objective.
- Environmental sources like soil and wastewater are rich reservoirs for identifying potent enzyme-producing microbial species.
Purpose of the Study:
- To isolate and select lipolytic fungi from environmental samples.
- To evaluate and compare the lipase-producing capabilities of selected fungal strains.
- To identify fungal candidates with high lipolytic activity for potential industrial use.
Main Methods:
- Submerged fermentation was employed for the cultivation and selection of lipolytic fungi.
- A total of 27 fungal strains were screened, comprising 20 isolates from dairy effluent and 7 from diesel-contaminated soil.
- Lipolytic activity was quantified, with enzyme units (U) defined as the amount of enzyme releasing 1 micromol of fatty acid per minute per mL of enzyme extract.
Main Results:
- Several fungal strains exhibited significant lipolytic activity.
- Penicillium E-3 demonstrated the highest lipolytic activity, reaching 2.81 U.
- Trichoderma E-19 and Aspergillus O-8 also showed notable activities of 2.34 U and 2.03 U, respectively.
- Maximum lipolytic activity for the selected fungi was observed on the 4th day of fermentation.
Conclusions:
- Environmental isolation is an effective strategy for discovering high-performing lipase-producing fungi.
- Penicillium E-3 is a promising candidate for industrial lipase production due to its superior activity.
- The optimal fermentation time for maximal lipase yield from these fungi is approximately 4 days.
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