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Aptamer-based biosensor for sensitive PDGF detection using diamond transistor.

A Rahim Ruslinda1, Shinya Tajima, Yoko Ishii

  • 1School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. ruslinda@toki.waseda.jp

Biosensors & Bioelectronics
|August 31, 2010
PubMed
Summary
This summary is machine-generated.

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This study demonstrates the first detection of platelet-derived growth factor (PDGF) using aptamer-functionalized diamond biosensors. These durable devices offer sensitive and repeatable PDGF detection without gate insulators.

Area of Science:

  • Nanotechnology and Materials Science
  • Biotechnology and Biosensing
  • Biochemistry and Molecular Biology

Background:

  • Platelet-derived growth factor (PDGF) is a crucial biomarker in various physiological and pathological processes.
  • Accurate and sensitive detection of PDGF is essential for early disease diagnosis and monitoring.
  • Existing biosensing platforms often face challenges with stability, sensitivity, and reusability.

Purpose of the Study:

  • To develop and demonstrate a novel biosensor for the direct detection of PDGF.
  • To utilize aptamers immobilized on a diamond surface within a solution-gate field-effect transistor (SGFET) architecture.
  • To evaluate the sensitivity, stability, and reusability of the developed diamond SGFET biosensor.

Main Methods:

  • Aptamers were covalently immobilized onto amine-functionalized diamond surfaces.

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  • The functionalized diamond was integrated into a solution-gate field-effect transistor (SGFET) configuration.
  • Detection of PDGF was performed by measuring shifts in the source-drain current upon analyte introduction.

    • Main Results:

    • A significant negative voltage shift of 31.7 mV was observed upon PDGF binding to the immobilized aptamers.
    • The biosensor demonstrated effective regeneration, with a positive voltage shift of 32.3 mV after SDS treatment, returning to the baseline.
    • The diamond SGFETs exhibited stable operation within a large potential window (>3.0 V) without the need for a gate insulator.

    Conclusions:

    • Diamond SGFETs functionalized with aptamers provide a sensitive and stable platform for PDGF detection.
    • The direct exposure of aptamer channels to electrolyte solutions enhances biosensor performance and simplifies fabrication.
    • The demonstrated durability and reusability indicate the potential of these biosensors for practical diagnostic applications.