Related Experiment Video
Updated: Jun 9, 2026
12:38
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
Published on: February 15, 2018
APAP and Alternative Titration Methods.
Omer Ahmed1, Sairam Parthasarathy
1Fellow, Pulmonary and Critical Care Medicine, Department of Medicine, University of Arizona, Tucson, AZ.
Sleep Medicine Clinics
|September 1, 2010
Summary
Alternative methods for positive airway pressure (PAP) titration, like auto-titrating PAP (APAP), are as effective as traditional methods for obstructive sleep apnea (OSA). However, implementation faces challenges like patient selection and provider knowledge.
Area of Science:
- Sleep Medicine
- Respiratory Therapy
Background:
- Positive airway pressure (PAP) therapy is a cornerstone treatment for obstructive sleep apnea (OSA).
- Traditional PAP titration involves manual adjustments by a sleep technician during polysomnography.
- Auto-titrating PAP (APAP) devices offer an alternative titration method with growing adoption.
Purpose of the Study:
- To review current alternative methods for PAP titration in patients with sleep-disordered breathing (SDB), focusing on OSA.
- To enhance understanding of emerging PAP titration technologies and their clinical application.
Main Methods:
- Review of recent prospective-randomized studies comparing alternative PAP titration methods with traditional manual titration.
- Analysis of factors influencing the adoption of alternative PAP titration techniques.
Main Results:
- Alternative PAP titration methods demonstrate comparable pressure determinations to traditional in-lab manual titrations.
- Key barriers to adoption include patient selection, device variability, reimbursement policies, and provider-specific factors.
Conclusions:
- Despite technological advancements and supporting evidence, significant barriers hinder the widespread implementation of alternative PAP titration methods.
- Further research and consensus-building are needed to overcome these obstacles and optimize PAP therapy delivery for SDB.
Related Concept Videos
01:08Titrimetric Methods: Types and Commonly Used Strategies
In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
01:23EDTA: Indirect and Alkalimetric Titration
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
01:19Precipitation Titration: Endpoint Detection Methods
In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
In the Volhard method, a standard excess of AgNO3 is first added to the...
01:30EDTA: Direct, Back-, and Displacement Titration
The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides and...
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides and...
01:31Potentiometric Titration: Overview
Potentiometric titration is a quantitative analytical technique that determines the concentration of an analyte by measuring the potential difference between the two electrodes in the solution. The endpoint of a potentiometric titration is the point at which there is a significant change in the potential difference. It occurs when the stoichiometric reaction between the analyte and the titrant is complete. The endpoint is usually determined graphically by plotting the measured potential...
01:18Effects of EDTA on End-Point Detection Methods
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...