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Updated: Jun 9, 2026

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance
Published on: August 26, 2025
Spectroscopic and theoretical study on the interaction between diperoxovanadate complexes and glycyl-histidine
Jun Zhang1, Xianyong Yu, Birong Zeng
1Department of Physics, Fujian Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, Fujian, China.
Abstract:
The interaction between diperoxovanadate complexes (K₃)[VO(O₂)₂(C₂O₄)]·H₂O and [VO(O₂)₂(D₂O)](-)/[VO(O₂)₂(HOD)](-)) and glycyl-histidine (abbr. GlyHis) in solution was studied by 1D NMR including variable-temperature NMR, 2D diffusion ordered spectroscopy (DOSY), HMQC and density functional theory (DFT) calculations. The results indicate that a pair of [VO(O₂)₂(GlyHis)](-) isomers is formed. The vanadium in the new species is coordinated by either the ɛ-N or δ-N in the imidazole ring. The relative ratio of the isomers was affected by temperature. Theoretical calculation results provide a reasonable explanation on the relative coordination capability of different nitrogen sites. Solvation effect is shown to be important for the reactivity of the interaction system.
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