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Updated: Jun 8, 2026

DNA Methylation: Bisulphite Modification and Analysis
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DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Investigation of DNA methylation by direct electrocatalytic oxidation

Po Wang1, Zhibin Mai, Zong Dai

  • 1School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.

Chemical Communications (Cambridge, England)
|September 11, 2010
PubMed

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Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...