Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 8, 2026

A Versatile Method of Patterning Proteins and Cells
09:57

A Versatile Method of Patterning Proteins and Cells

Published on: February 26, 2017

Programmable microfluidic patterning of protein gradients on hydrogels.

Simone Allazetta1, Steffen Cosson, Matthias P Lutolf

  • 1Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne Station 15 Bldg. AI 1109, CH-1015 Lausanne, Switzerland.

Chemical Communications (Cambridge, England)
|September 11, 2010
PubMed
Summary

Computer-controlled hydrodynamic flow focusing precisely creates custom protein gradients on synthetic hydrogel surfaces. This technique enables versatile applications in biomaterials and cell culture studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing mechanobiology from single molecules to complex cellular systems.

Nature nanotechnology·2026
Same author

Engineered intestinal crypt geometry uncovers YAP1-dependent fetal-to-adult transition.

Cell stem cell·2026
Same author

A microphysiological human mini-bladder reveals urine-urothelium interplay in tissue resilience and UPEC recurrence in urinary tract infections.

Nature communications·2026
Same author

Chemotherapy-driven intestinal dysbiosis and indole-3-propionic acid rewire myelopoiesis to promote a metastasis-refractory state.

Nature communications·2025
Same author

Bioengineering mini-colons for ex vivo colorectal cancer research.

Nature protocols·2025
Same author

An extracellular matrix niche secreted by epithelial cells drives intestinal organoid formation.

Developmental cell·2025

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cellular Engineering

Background:

  • Generating controlled protein gradients is crucial for understanding cell behavior.
  • Existing methods often lack precision in shape and reproducibility.
  • Soft synthetic hydrogels offer tunable platforms for biological interfaces.

Purpose of the Study:

  • To develop a novel method for creating user-defined protein gradients on hydrogel surfaces.
  • To demonstrate the versatility and precision of the developed technique.
  • To enable advanced applications in biomimetics and tissue engineering.

Main Methods:

  • Utilized computer-controlled hydrodynamic flow focusing.
  • Engineered tethered protein gradients with arbitrary shapes.

More Related Videos

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel
13:28

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel

Published on: August 8, 2017

Related Experiment Videos

Last Updated: Jun 8, 2026

A Versatile Method of Patterning Proteins and Cells
09:57

A Versatile Method of Patterning Proteins and Cells

Published on: February 26, 2017

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel
13:28

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel

Published on: August 8, 2017

  • Fabricated soft synthetic hydrogels as the substrate material.

    • Main Results:

    • Successfully generated protein gradients of user-defined shapes on hydrogel surfaces.
    • Demonstrated precise control over gradient morphology and placement.
    • Established a robust platform for surface protein patterning.

    Conclusions:

    • Computer-controlled hydrodynamic flow focusing is an effective technique for creating complex protein gradients.
    • This method provides a versatile tool for fabricating advanced biomaterial surfaces.
    • The technology has significant potential for applications in cell-based assays and regenerative medicine.