Related Experiment Video
Updated: Jun 8, 2026
08:06
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag
Kalyan K Sadhu1, Shin Mizukami, Shuji Watanabe
1Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Chemical Communications (Cambridge, England)
|September 11, 2010
Abstract:
The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.
