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Updated: Jun 8, 2026

Quantification of Hypopigmentation Activity In Vitro
Published on: March 6, 2019
Melanogenesis inhibition due to NADH
Francis Garcia-Molina1, Joseph Louis Munoz-Munoz, Mary Garcia-Molina
1GENZ: Investigative Group for Enzymology, Department of Biochemistry and Molecular Biology A, Faculty of Biology, University of Murcia, Murcia, Spain.
Abstract:
The effect of NADH on melanogenesis under aerobic conditions involves three types of reaction: (a) acting as tyrosinase substrate (a competitive substrate of L-tyrosine and L-DOPA), (b) irreversible inactivation acting as a suicide substrate of tyrosinase, and (c) non-enzymatic reduction of o-dopaquinone by NADH. Under anaerobic conditions, NADH irreversibly inhibits the enzymatic forms met-tyrosinase and deoxy-tyrosinase. In this paper, we kinetically characterize this coenzyme as it acts as a tyrosinase suicide substrate and propose a kinetic mechanism to explain its oxidation by tyrosinase. In addition, the compound is characterized as an irreversible inhibitor of met-tyrosinase and deoxy-tyrosinase.
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