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A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

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Exploring zebrafish genomic, functional and phenotypic data using ZFIN.

Sridhar Ramachandran¹, Barbara Ruef, Christian Pich

¹University of Oregon, Eugene, Oregon, USA.

Current Protocols in Bioinformatics

|September 14, 2010

Summary

The Zebrafish Information Network (ZFIN) offers a comprehensive web resource for zebrafish data, including genomic, genetic, and developmental information. It provides tools to search, visualize, and analyze this crucial biological data.

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Area of Science:

* Developmental Biology
* Genomics
* Bioinformatics

Background:

* The Zebrafish Information Network (ZFIN) is a vital web resource for zebrafish data.
* It integrates genomic, genetic, developmental, and phenotypic information.
* Data is sourced from scientific literature and direct laboratory submissions.

Purpose of the Study:

* To outline methods for searching, visualizing, and analyzing ZFIN data.
* To highlight the utility of ZFIN for researchers studying zebrafish.
* To introduce key ZFIN tools and resources.

Main Methods:

* Utilizing ZFIN's web-based search and display tools.
* Exploring data types including genes, gene expression, mutants, morphants, and transgenics.
* Leveraging ZFIN's GBrowse genome viewer, BLAST functionality, and wiki resources.

Main Results:

* Demonstrated accessibility of diverse zebrafish data through ZFIN.
* Showcased methods for analyzing genes, developmental processes, and phenotypic variations.
* Highlighted the integration of ZFIN with other bioinformatics resources.

Conclusions:

* ZFIN serves as a central hub for zebrafish research data.
* The platform facilitates efficient data retrieval and analysis for biological discovery.
* ZFIN's tools are essential for researchers in genetics, development, and comparative genomics.