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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Basic design of MRM assays for peptide quantification.

Andrew James¹, Claus Jorgensen

¹Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|September 15, 2010

Summary

This summary is machine-generated.

Multiple reaction monitoring (MRM) offers a sensitive and robust method for detecting proteins. This guide details designing and optimizing MRM workflows for proteomics research and quantification.

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Area of Science:

Proteomics
Analytical Chemistry
Biochemistry

Background:

Mass spectrometry is increasingly accessible for research.
There is a growing need for sensitive and reproducible protein detection assays.
Multiple Reaction Monitoring (MRM) is a selective and robust assay for biomolecule monitoring.

Purpose of the Study:

To introduce the principles of designing and optimizing MRM workflows.
To provide examples of MRM workflows for proteomic samples.
To offer guidance for researchers interested in MRM quantification.

Main Methods:

The chapter focuses on the principles and optimization of MRM workflows.
Examples of MRM workflows for standard proteomic samples are presented.

Main Results:

MRM assays are highly selective, sensitive, and robust for biomolecule detection.
MRM has expanded from drug testing to proteomics, biomarker discovery, and systems biology.

Conclusions:

MRM is a valuable technique for protein quantification in proteomics.
This chapter serves as a guide for implementing and optimizing MRM assays.