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Related Concept Videos

Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
Viral Hepatitis I: Introduction01:28

Viral Hepatitis I: Introduction

Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion of food...

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Related Experiment Video

Updated: Jun 8, 2026

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

[Cell culture system for hepatitis E virus].

Hiroaki Okamoto1

  • 1Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi-ken 329-0498, Japan. hokamoto@jichi.ac.jp

Uirusu
|September 21, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed an efficient cell culture system for hepatitis E virus (HEV) propagation. This system allows for efficient replication of various HEV strains from feces and sera, aiding further research.

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"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
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"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

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Last Updated: Jun 8, 2026

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
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A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
10:25

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

Area of Science:

  • Virology
  • Hepatology
  • Infectious Diseases

Context:

  • Hepatitis E virus (HEV) propagation in vitro has been historically inefficient.
  • Previous studies utilized primary hepatocytes or cell lines with limited success.
  • High-titer inocula from Japanese patients (genotype 3 and 4 HEV) were used.

Purpose:

  • To establish an efficient cell culture system for HEV propagation.
  • To enable serial passage and study of different HEV strains.
  • To investigate the role of HEV ORF3 protein in viral egress.

Summary:

  • An efficient cell culture system for HEV was developed using PLC/PRF/5 and A549 cells with high-titer fecal inocula.
  • The system achieved high HEV loads (10^8 copies/ml) and allowed for over six serial passages.
  • A full-length infectious cDNA clone and an ORF3-deficient mutant were utilized to study viral replication and egress.
  • The ORF3 protein was identified as crucial for virion egress and associated with lipids on released particles.
  • Efficient propagation of various HEV strains from blood circulation was also achieved.

Impact:

  • Facilitates efficient propagation of diverse HEV strains from clinical samples (feces, sera).
  • Enables in-depth studies on strain-specific viral replication mechanisms.
  • Provides a robust platform for developing antiviral strategies and diagnostics for Hepatitis E.