Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revealing Hidden Dynamics of Hydrogel-Based Desalination with <sup>23</sup>Na Nuclear Magnetic Resonance.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same author

Mapping mRNA Localization and Internal Structure in Lipid Nanoparticles through Solid-State Dynamic Nuclear Polarization NMR and Proton Spin-Diffusion Modeling.

Small methods·2026
Same author

Robust single-scan ultraselective NMR.

Chemical communications (Cambridge, England)·2026
Same author

Lithium Nuclear Spin Polarization Lifetimes as Sensitive Reporters of Battery Electrolyte Degradation.

ACS omega·2026
Same author

Over four minutes of pyruvate T<sub>1</sub> using chemically and physically induced deceleration of relaxation.

Nature communications·2026
Same author

Fast and flow-compatible pseudo-3D diffusion NMR.

Chemical communications (Cambridge, England)·2026

Related Experiment Video

Updated: Jun 8, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Observation of NMR noise from solid samples.

Judith Schlagnitweit1, Jean-Nicolas Dumez, Martin Nausner

  • 1Johannes Kepler University in Linz, Institute of Organic Chemistry, Altenbergerstraße 69, 4040 Linz, Austria.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|September 21, 2010
PubMed
Summary
This summary is machine-generated.

Proton nuclear magnetic resonance (NMR) noise signals can now be acquired from solid samples. This advancement allows for improved signal-to-noise ratios in solid-state NMR experiments.

More Related Videos

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
12:47

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

Related Experiment Videos

Last Updated: Jun 8, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
12:47

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Materials Science
  • Analytical Chemistry

Background:

  • Nuclear Magnetic Resonance (NMR) is a powerful technique for structural elucidation.
  • Traditionally, NMR requires radio frequency excitation to generate spectra.
  • Acquiring NMR signals from solid samples presents unique challenges compared to liquids.

Purpose of the Study:

  • To demonstrate the feasibility of obtaining proton NMR noise signals from solid samples.
  • To explore the applicability of this technique under different experimental conditions.
  • To adapt and optimize a tuning procedure for solid-state NMR noise acquisition.

Main Methods:

  • Acquisition of proton NMR noise signals from solid samples without radio frequency excitation.
  • Experimental validation under static and magic-angle spinning (MAS) conditions.
  • Application of a probe tuning procedure optimized for NMR noise characteristics.

Main Results:

  • Successful detection of proton NMR noise signals in solid samples.
  • Demonstration of signal acquisition under both static and MAS conditions.
  • Validation of a tuning procedure to enhance signal-to-noise ratios in ¹H-MAS experiments.

Conclusions:

  • Proton NMR noise spectroscopy is a viable technique for solid samples.
  • The described tuning procedure effectively optimizes signal-to-noise ratios for solid-state NMR.
  • This method offers a new avenue for characterizing solid materials using NMR.