Related Experiment Video
Updated: Jun 8, 2026

Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Comparative physicochemical and biocompatible properties of radiopaque dicalcium silicate cement and mineral trioxide
Ting-Yi Chiang1, Shinn-Jyh Ding
1Department of Dental Laboratory Technology, Central Taiwan University of Science and Technology, Taichung City, Taiwan.
Introduction:
The purpose of this study was to comparatively examine physicochemical and biocompatible properties of 20 wt% bismuth oxide (Bi(2)O(3))-containing dicalcium silicate cement and white-colored mineral trioxide aggregate (WMTA).
Methods:
The radiopacity, setting time, diametral tensile strength, pH value, morphology, and phase composition of the cements with and without Bi(2)O(3) were measured after mixing powders with water. Cement biocompatibility was evaluated by incubating the cement specimens with MG63 human osteoblast-like cells.
Results:
The addition of Bi(2)O(3) to the cement led to a significant increase (p < .05) in the setting time of 24 minutes. It was accompanied by a small decrease in the pH value but without adversely affecting diametral tensile strength. The radiopacity value of the Bi(2)O(3)-containing cement was equivalent to 7.3 mm of aluminum, which was significantly higher (p < 0.05) than pure cement without Bi(2)O(3) (1.1 mm of aluminum) but lower (p < .05) than WMTA (9.3 mm of aluminum). However, it was greater than 3 mm of aluminum, which is the value recommended by ISO 6876/2001 standards. MG63 cell viability cultured on Bi(2)O(3)-containing cement was higher than that cultured on WMTA at all culture times.
Conclusions:
The dicalcium silicate cement with 20 wt% Bi(2)O(3) showed shortened setting time and good biocompatibility and thus may have the potential to be a root-end filling material alternative to MTA.
Related Concept Videos
Hydration of Cement
Aggregate Cement Ratio
Mortar Properties
Strength and Heat of Hydration
The heat of hydration for each cement compound is significant; for instance, tricalcium aluminate (C3A) and...
Soundness of Cement
Alkali Aggregate Reaction in Concrete
